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KMID : 0545120110210070675
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 7 p.675 ~ p.678
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Improvement of Transformation Efficiency by Strategic Circumvention of Restriction Barriers in Streptomyces griseus
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Hirokazu Suzuki
Shunji Takahashi
Hiroyuki Osada
Ken-ichi Yoshida
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Abstract
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DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-GC5mCGGC-3' and 5'-GAG5mCTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.
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KEYWORD
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DNA methylation, DNA restriction, host-mimicking DNA, Streptomyces griseus
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